Journal: Behavioural Pharmacology

Article Title: Naltrexone treatment improves anxiety- and depression-like behavior in alcohol-exposed mice

doi: 10.1097/FBP.0000000000000876

Figure Lengend Snippet: Naltrexone treatment ameliorates microglial activation in alcohol-exposed mice. (a) Immunofluorescence images of microglia labeled with IBA1 (green) in the BLA region (DAPI, blue, for nuclear counterstaining); each image is a representative result from n = 4 independent mice, with images in each treatment group derived from brain tissues of different mice and corresponding coronal sections of the same brain region to ensure intergroup comparability ( n = 4 each group). (b) Quantification of microglial cell numbers in the BLA region ( F = 10.53, DF = 11, P < 0.01; n = 4 each group) * P < 0.05, ** P < 0.01, *** P < 0.001. BLA, basolateral amygdala; DAPI, 4',6-diamidino-2-phenylindole.

Article Snippet: Sections were blocked (5% BSA + 0.5% Triton X-100 in PBS, 1 h, room temperature) and incubated with anti-ionized calcium-binding adapter molecule 1 (IBA1) primary antibody (1 : 500, Proteintech, China, 4°C overnight), followed by Alexa Fluor 488-conjugated secondary antibody (Thermo Fisher Scientific, Waltham, USA, 1 h, room temperature).

Techniques: Activation Assay, Immunofluorescence, Labeling, Derivative Assay